Validation of Fao/iaea/panaftosa Elisa Kit for the Detection of Antibodies against Foot-and-mouth Disease Virus in Venezuela
نویسندگان
چکیده
VALIDATION OF FAO/IAEA/PANAFTOSA ELISA KIT FOR THE DETECTION OF ANTIBODIES AGAINST FOOTAND-MOUTH DISEASE VIRUS IN VENEZUELA. A liquid phase blocking ELISA (LPBE) supplied by FAO/IAEA/PANAFTOSA has been evaluated for the qualitative and quantitative detection of specific antibodies to "O" and "A" serotypes of foot-and-mouth disease (FMD). A total of 240 bovine sera were analyzed. 120 sera from non-infected and non-vaccinated cattle were tested in a screening test showing a specificity of 99,2%. 120 from vaccinated cattle were tested in a titration assay giving a sensitivity of 99,2%. For serotype “O” the titration test showed a protection of 80% and for serotype "A" 75,6%. Antibody titers fluctuated between >112 and >1250 which indicates protection.
منابع مشابه
Development of an Indirect Enzyme-linked Immunosorbent Assay to Detect Antibodies against Serotype A2013 of Foot and Mouth Disease Virus in Cattle
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